There are currently 10 Bosses in the game. A Boss usually has to be beaten to progress to the next Chapter. When a boss is defeated for the first time, their respective card is collected.
Here is the full list and where they have to be fought:
| Boss | Picture | Chapter | Map(s) |
|---|---|---|---|
| Diggy Mole | 1 | Ruins/Oasis/Quarry | |
| Dr. Turret | 1 | Ruins/Oasis/Quarry | |
| Billy Boom | 1 | Ruins/Oasis/Quarry | |
| Elite Gang | 1 | Scrapyard | |
| El'Moustiko | 1 | The Pit | |
| Sir Catercoaster | 2 | Energy Center | |
| The Judgeball | 2 | Energy Center | |
| Duke Nuker | 2 | Fusion Core | |
| Beetle Royale | 3 | District XIII | |
| Uncle Jim | 3 | District XIII | |
| IRIS | 4 | The Moon |
While the Elite Gang is not listed as a Boss in the Museum, it functions identically to the other and is included here for completion's sake.